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. 2022 Nov 5;13:6688. doi: 10.1038/s41467-022-34558-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Leukemogenic potential of MLL-AF4 sPTRS in vivo. Murine HSPCs were transduced with retrovirus for the MLL-AF4 sPTRS construct and transplanted into syngeneic mice. MLL-AF4 sPTRS, n = 30. The B/M-MPAL and T-ALL cases were excluded as retrovirus genome integration was not detected (Supplementary Fig. 3). The MLL-AF4 and MLL-mAf4 data (Fig. 1b) are shown for comparison. b The immunophenotype of MLL-AF4 sPTRS-mediated leukemia. Bone marrow cells from mice with MLL-AF4 sPTRS-mediated leukemia were analyzed via flow cytometry for the indicated markers. Unstained control is indicated in blue and stained sample indicated in red. c The expression of myeloid markers, Gr1 and Cd11b, in AML cells of MLL-AF4 sPTRS-leukemia. MLL-mAF4- and MLL-ENL-mediated AMLs were included for comparison. d qRT-PCR using bone marrow cells from mice with MLL-AF4 sPTRS-mediated leukemia. Expression levels normalized to Gapdh are shown relative to those of HSPCs. Data are presented as the mean ± SD of PCR triplicates. See also Supplementary Fig. 3. Source data are provided as a Source Data file.