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. 2022 Nov 5;13:6688. doi: 10.1038/s41467-022-34558-1

Fig. 5. IGF2BP3 is responsible for the post-transcriptional inactivation of MLL-AF4.

Fig. 5

a Transforming ability of MLL-AF4 sAU13 in the absence of Igf2bp3. The MLL-AF4 construct carrying synonymous mutations at AU1 and 3 was examined for transformation of HSPCs under an ex vivo myeloid condition with co-transduction of a knockout construct for Igf2bp3. CFUs are shown as in Fig. 1a, with images of representative colonies (n = 4). Hoxa9 expression during fourth-round passage is shown (n = 3). b Western blotting of MLL-AF4 sAU13 mutant and endogenous murine IGF2BP3 in immortalized cells. Western blotting was performed on two biological replicates. c Inhibition of MLL-AF4-immortalized cell proliferation by rescuing human IGF2BP3 expression. Cells immortalized by MLL-AF4 sAU13 were transduced with a lentivirus carrying IGF2BP3 and GFP. The ratio of GFP-positive cells was monitored on days 3 and 7 (n = 4). Data are presented as the mean ± SD of indicated biologically independent replicates (a, c). P-value was calculated by two tailed T-test (c). See also Supplementary Fig. 5. Source data are provided as a Source Data file.