Fig. 6. RBP complex inhibits translation of MLL-AF4.

a Schematic representation of the reporter constructs for post-transcriptional regulation. b Subcellular localization of GFP and RFP reporters in 293 T cells. c Relative fluorescent signals of GFP and RFP reporters. The fluorescent intensities were analyzed using ImageJ software and expressed as the GFP/RFP ratio (n = 7: WT, hPTRS, mPTRS, sPTRS; n = 4: hPTRS sAU123, mPTRS hAU123, STOP-hPTRS). Data are presented as the mean ± SD of indicated biologically independent replicates. P-value was calculated by one-way ANOVA followed by Tukey’s test. d Western blotting of the GFP/RFP reporter proteins in 293 T cells transiently expressing the constructs. GFP and RFP proteins were detected using FLAG and HA antibodies, respectively. e Co-localization of GFP reporter and RNA-binding proteins. The GFP-tagged hPTRS reporter and mCherry-tagged RBPs constructs were co-transfected in 293 T cells. Nuclei were stained with Hoechst 33342. f Association of ribosomal proteins with GFP reporters. FLAG-tagged GFP reporters transiently expressed in 293 T cells were analyzed via immunoprecipitation-western blotting with anti-FLAG antibody. Endogenous proteins were detected using specific antibodies. Western blotting was performed on two biological replicates (d, f). See also Supplementary Fig. 6. Source data are provided as a Source Data file.