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. 2022 Nov 5;13:6681. doi: 10.1038/s41467-022-34363-w

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a WMISH for ectodermin; lateral views with the animal pole to the top; asterisk marks the animal pole with loss of ectodermin expression; embryos with absent expression are quantified in c; CR CRISPR, Nfd nifedipine. b WMISH for myf5: vegetal views with dorsal to the top; arrowhead marks loss of expression; embryos with abnormal expression are quantified in d. c, d Percentages of embryos with abnormal ectodermin (c) and myf5 (d) expression. Graphs depict mean ± SEM; p-values are in c: (kcnh6MO+DMSO vs DMSO) = 2.88e-006, (kcnh6MO+Nfd vs kcnh6MO+DMSO) = 4.59e-002, (kcnh6MO+cacna1cCR vs kcnh6MO) = 1.53e-002, (kcnh6MO+cacna1gCR vs kcnh6MO) > 9.99e-001 (ns), (kcnh6CR+DMSO vs DMSO) = 3.50e-007, (kcnh6CR+Nfd vs kcnh6CR+DMSO) = 1.20e-002; in d: p-values are (kcnh6MO+DMSO vs DMSO) = 4.07e-015, (kcnh6MO+Nfd vs kcnh6MO+DMSO) = 8.92e-005, (kcnh6MO+cacna1cCR vs kcnh6MO) = 4.78e-005, (kcnh6MO+cacna1gCR vs kcnh6MO) = 2.24e-001 (ns), (kcnh6CR+DMSO) = 2.04e-014, (kcnh6CR+Nfd) = 1.45e-003; two-sided Fisher’s exact test; total embryo numbers (N) in the graphs are from at least 2 independent experiments; Key for asterisks: *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001, ns nonsignificant for p > 0.05. Source data are provided as a Source Data file.