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. 2022 Nov 5;13:6681. doi: 10.1038/s41467-022-34363-w

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a–c WMISH for pluripotency markers pou5f3.3, ventx1.2, and differentiation marker ectodermin in stage 10 embryos, depolarized by kcnh6 depletion (kcnh6CR) or high K+ (hiK), and treated with vehicle (DMSO) or rapamycin (Rapa) as indicated; views are a animal pole; b, c lateral view with dorsal to the right; AP animal pole, VMZ ventral marginal zone. d–f Quantification of stage 10 embryos with present (+) or absent (−) expression of markers pou5f3.3 (d), ventx1.2 (e), and ectodemin (f) in the animal pole area. p-values in d are: (kcnh6CR+Rapa vs kcnh6CR+DMSO) = 3.34e-087 and (hiK+Rapa vs hiK+DMSO) = 2.89e-047, in e: (kcnh6CR+Rapa vs kcnh6CR+DMSO) = 1.60e-110 and (hiK+Rapa vs hiK+DMSO) = 1.93e-050, and in f: (kcnh6CR+Rapa vs kcnh6CR+DMSO) = 3.38e-040 and (hiK+Rapa vs hiK+DMSO) = 4.89e-02; two-sided Fisher’s exact test; total embryo numbers (N) in the graphs were collected over 3 independent experiments for pou5f3.3 and ventx1.2 and over 4 independent experiments for ectodermin; key for asterisks: ****p ≤ 0.0001. Source data are provided as a Source Data file.