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. 2022 Nov 5;8:448. doi: 10.1038/s41420-022-01244-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A HEL cells were treated with indicated doses of UM171 and subjected to western blot for P21^CIP1 and PIM1 expression. B, C HEL cells were treated with UM171, LGH447, and UM171 + LGH447 for 24 hours and subjected to Q-RT-PCR (B) or western blot (C) analysis for the indicated genes. D, E HEL cells were treated with UM171, GSK-LDS1 (10 μM), and P21^CIP1 levels were determined by Q-RT-PCR (D) and western blotting (E). F Cell cycle arrest of HEL cells treated with UM171, LGH447 and UM171 + LGH447. Right panel shows the percentage of cells in different phases of the cell cycle.