FIG. 3.

Immunoblot analysis of recombinant and native pypAg-1. (A) Immunoblot of purified recombinant pAg-1N (lane 1 and 3) and pAg-1C (lanes 2 and 4) probed with sera from mice immunized with the pAg preparation of P. yoelii blood-stage antigens (lanes 3 and 4) or with sera from adjuvant control mice (lanes 1 and 2). (B) Immunoblot of P. yoelii total blood-stage antigens (lanes 1 and 3) or particulate blood-stage antigens (lanes 2 and 4) probed with preimmune rabbit serum (lanes 1 and 2) or rabbit antiserum raised against recombinant pAg-1N (lanes 3 and 4). Molecular mass markers are indicated.