FIG. 3.

Effect of PTK blockade on IL-8 production. Monocytes and PMN were incubated with herbimycin A (10 μM) for 1 h prior to LPS (1 μg/ml) or LAMPf (1 μg/ml) stimulation. DMSO (1%) was used as the solvent control. The IL-8 level was measured by ELISA 18 h after stimulation and normalized to cells that received no treatment prior to stimulation. Data presented are means ± SEM of four and three distinct experiments for monocytes and PMN, respectively.