FIG. 4.

Effect of ERK1/2 and p38 pathway-specific inhibitors on IL-8 production by human monocytes and PMN. Cells were treated for 1 h with either PD-98059 (MEK1 inhibitor) or SB203580 (p38 inhibitor) at different concentrations and then stimulated with either LAMPf (1 μg/ml) (A) or LPS (1 μg/ml) (B). DMSO (1%) was used as the solvent control. The IL-8 level was measured by ELISA 18 h after stimulation and normalized to cells that received no treatment prior to stimulation. Results are means ± SEM of four and three separate experiments for monocytes and PMN, respectively.