FIG. 5.

Effect of CAPE on NF-κB transactivation mediated by LAMPf. THP-1 cells were transiently transfected with an NF-κB- or AP-1-driven luciferase (luci) reporter plasmid. Transfected cells were stimulated with LAMPf (1 μg/ml) for 6 h prior to cell lysis. Luciferase activity was assessed in stimulated and unstimulated (control [CTRL]) cells and normalized to protein content. To assess the effect of CAPE, transfected cells were preincubated with CAPE at the indicated concentration for 1 h and then stimulated with LAMPf and analyzed for luciferase activity as indicated above. Assays were performed in duplicate, and data presented are means ± SEM of three independent experiments.