Skip to main content
. 2022 Jun 27;43(42):4496–4511. doi: 10.1093/eurheartj/ehac337

Figure 5.

Figure 5

Circ-INSR is regulated by Brca1. (A) Prediction of the upstream regulator of Circ-INSR using the RegRNA2.0 tool. (B) Relative expression of Brca1 in mouse heart tissue with or without doxorubicin treatment (n = 6 mice per group). (C) Relative expression of Brca1 in HL-1 cells treated with Brca1-siRNA compared with Scramble siRNA (n = 7, from two independent experiments). (D) Relative expression of Circ-INSR and INSR in HL-1 cells treated with Brca1-siRNA compared with Scramble siRNA (n = 7, from two independent experiments). (E) Relative expression of Circ-INSR and INSR in pcDNA-Circ-INSR transfected HL-1 cells co-treated with Brca1-siRNA compared with control transfected with pcDNA-Empty and Scramble siRNA (n = 7, from two independent experiments, one-way analysis of variance performed within Control, pcDNA-Circ-INSR + Scramble siRNA, and pcDNA-Circ-INSR + Brca1-siRNA). All quantitative data are presented as mean ± standard error mean, and an unpaired two-tailed t-test was performed to calculate significance between two groups and one-way analysis of variance with post hoc Tukey test was used to calculate significance between ≥3 groups wherever required. P < 0.05 was considered as statistically significant. exp., expression; FC, fold change; Rel., relative.