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. 2022 Nov 7;3(4):101872. doi: 10.1016/j.xpro.2022.101872

Figure 1.

Figure 1

Typical morphology of hPSC colonies cultured on VTN-N-coated plates with E8 medium

(A) Representative bright field images of the hiPSC line CBiPS1sv-4F-40 and the hESC line ES[4] at day 2, day 3 and day 5 after passaging. After 5 days in culture, hPSC colonies show nearly 80% confluency.

(B) Representative bright field image taken 24 h after thawing an hPSC cryovial that shows dead floating hPSCs because of a failed thawing procedure.

(C) Representative bright field image taken 48 h after hPSC passaging that shows very few small cell clusters indicating a failed passage procedure.

(D) Representative bright field images of hPSC colonies with spontaneous differentiation. Yellow arrows indicate differentiated cells. Scale bars in (A-D), 100 μm.