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. 2022 Nov 7;3(4):101872. doi: 10.1016/j.xpro.2022.101872

Figure 5.

Figure 5

Immunofluorescence analysis of kidney organoids after exposure to diabetogenic-like culture conditions

(A) Schematic overview of the procedure to induce control or diabetic kidney organoids.

(B) Representative bright field images of control or diabetic kidney organoids. Scale bars, 100 μm.

(C) Representative confocal images of control or diabetic kidney organoids analyzed by whole mount immunofluorescence for the detection of ACE2 (green), LTL (gray) and DAPI (blue). Scale bars, 250 μm, 100 μm (high magnification views).

(D) Example of representative confocal images that show the detection of ACE2 (green), LTL (gray) and DAPI (blue) by immunofluorescence in an organoid paraffin section. Scale bars, 250 μm, 100 μm (high magnification view). Yellow arrowheads in (C-D) indicate examples of LTL positive proximal tubule-like structures with ACE2 expressing cells.