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Intermediate Mesoderm Induction Medium
| Reagent | Final concentration | Volume |
|---|---|---|
| Complete Advanced RPMI 1640 Medium | N/A | 15 mL |
| FGF9 (50 μg/mL) | 200 ng/mL | 60 μL |
| Activin A (50 μg/mL) | 10 ng/mL | 3 μL |
| Heparin (5 mg/mL) | 1 μg/mL | 3 μL |
| Total | N/A | 15 mL |
Prepare fresh, bring to 20°C–22°C prior to use. For heparin, use an intermediate stock solution at 5 mg/mL by diluting the 50 mg/mL stock at 1:10 ratio.
