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. 2022 Nov 7;3(4):101872. doi: 10.1016/j.xpro.2022.101872

Kidney Organoid Differentiation Medium

Reagent Final concentration Volume
Complete Advanced RPMI 1640 Medium N/A 40 mL
FGF9 (50 μg/mL) 200 ng/mL 160 μL
Heparin (5 mg/mL) 1 μg/mL 8 μL
Total N/A 40 mL

Prepare fresh, bring to 20°C–22°C prior to use. For heparin, use an intermediate stock solution at 5 mg/mL by diluting the 50 mg/mL stock at 1:10 ratio.