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Stock solution of D-Glucose (1 M)
| Reagent | Final concentration | Amount |
|---|---|---|
| D-Glucose | 1 M | 18.0 g |
| Distilled Water, Cell culture grade | N/A | 100 mL |
| Total | 1 M | 100 mL |
Mix 18 g of glucose with 80 mL of cell culture grade distilled water using magnetic stirring until complete dissolution. Then, bring to a total volume of 100 mL with cell culture grade distilled water. Filter the solution using a 0.22 μm pore size syringe filter and store at 4°C, use within 2 months. Do not autoclave glucose solutions.
