Reagent | Final concentration | Volume (mL) |
---|---|---|
DMEM (High Glucose) | N/A | 485 |
P/S (10.000 U/mL) | 1% | 5 |
FBS | 2% | 10 |
Total | N/A | 500 |
Store at 4°C, use within 6 weeks, bring to 37°C prior to use.
Reagent | Final concentration | Volume (mL) |
---|---|---|
DMEM (High Glucose) | N/A | 485 |
P/S (10.000 U/mL) | 1% | 5 |
FBS | 2% | 10 |
Total | N/A | 500 |
Store at 4°C, use within 6 weeks, bring to 37°C prior to use.