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. 2022 Nov 7;3(4):101872. doi: 10.1016/j.xpro.2022.101872

Hyperglycemic Medium (25 mM Glucose)

Reagent Final concentration Volume (mL)
DMEM (glucose free) N/A 477.5
P/S (10.000 U/mL) 1% 5
GlutaMAX (200 mM) 1% 5
D-Glucose (1 M) 25 mM 12.5
Total N/A 500

Store at 4°C, use within 6 weeks, bring to 37°C prior to use.