Reagent | Final concentration | Volume (mL) |
---|---|---|
DMEM (glucose free) | N/A | 477.5 |
P/S (10.000 U/mL) | 1% | 5 |
GlutaMAX (200 mM) | 1% | 5 |
D-Glucose (1 M) | 25 mM | 12.5 |
Total | N/A | 500 |
Store at 4°C, use within 6 weeks, bring to 37°C prior to use.
Reagent | Final concentration | Volume (mL) |
---|---|---|
DMEM (glucose free) | N/A | 477.5 |
P/S (10.000 U/mL) | 1% | 5 |
GlutaMAX (200 mM) | 1% | 5 |
D-Glucose (1 M) | 25 mM | 12.5 |
Total | N/A | 500 |
Store at 4°C, use within 6 weeks, bring to 37°C prior to use.