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Hyperglycemic Medium (25 mM Glucose)
| Reagent | Final concentration | Volume (mL) |
|---|---|---|
| DMEM (glucose free) | N/A | 477.5 |
| P/S (10.000 U/mL) | 1% | 5 |
| GlutaMAX (200 mM) | 1% | 5 |
| D-Glucose (1 M) | 25 mM | 12.5 |
| Total | N/A | 500 |
Store at 4°C, use within 6 weeks, bring to 37°C prior to use.
