Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Plaque Assay Overlay Medium
| Reagent | Final concentration | Volume (mL) |
|---|---|---|
| EMEM/14% FBS Medium | N/A | 50 |
| Agarose solution (1%) | 0.5% | 50 |
| Total | N/A | 100 |
Equilibrate the EMEM+14% FBS at 20°C–22°C. Prepare the plaque assay overlay medium just before adding the overlay on cells by mixing at 1:1 dilution ratio EMEM+14%FBS (at 20°C–22°C) and 1% agarose solution (at 55°C). Use immediately to overlay the cell monolayers.
