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. 2022 Nov 7;3(4):101872. doi: 10.1016/j.xpro.2022.101872

Crystal Violet staining solution (1% w/v)

Reagent Final concentration Amount
Crystal violet 1% 1 g
Absolute Ethanol 20% 20 mL
Formaldehyde (37%) 25% 25 mL
PBS 1× N/A 55 mL
Total 1% 100 mL

Store at 20°C–22°C protected from light, use within 4 months. Mix before each use.