Reagent | Final concentration | Amount |
---|---|---|
Crystal violet | 1% | 1 g |
Absolute Ethanol | 20% | 20 mL |
Formaldehyde (37%) | 25% | 25 mL |
PBS 1× | N/A | 55 mL |
Total | 1% | 100 mL |
Store at 20°C–22°C protected from light, use within 4 months. Mix before each use.
Reagent | Final concentration | Amount |
---|---|---|
Crystal violet | 1% | 1 g |
Absolute Ethanol | 20% | 20 mL |
Formaldehyde (37%) | 25% | 25 mL |
PBS 1× | N/A | 55 mL |
Total | 1% | 100 mL |
Store at 20°C–22°C protected from light, use within 4 months. Mix before each use.