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. 2022 Aug 1;30(11):3414–3429. doi: 10.1016/j.ymthe.2022.07.019

Figure 2.

Figure 2

USP19 regulates survivin protein stability

(A) The effect of USP19 on endogenous survivin protein expression was determined in HCT116 cells. (B) Exogenous protein levels of survivin in HCT116 cells were analyzed upon transfection with increasing concentrations of FLAG-USP19, along with a constant amount of FLAG-survivin. (C) The effect of the catalytic mutant form of USP19 (USP19C506A) on endogenous survivin protein was analyzed upon transfection with increasing concentrations of USP19C506A. (D) The effect of USP19C506A on exogenous survivin protein was analyzed upon transfection with increasing concentrations of USP19C506A, along with a constant amount of FLAG-survivin in HCT116 cells. Western blot analysis was performed with the indicated antibodies. (E and F) HCT116 cells were transfected with indicated plasmids to check the reconstitution effect of USP19 in USP19-depleted cells on survivin by western blot analysis at an (E) endogenous or (F) exogenous level. (G and H) Interactions between (G) endogenous and (H) exogenous USP19 and survivin were analyzed in HCT116 cells. Cell lysates were immunoprecipitated with USP19- or survivin-specific antibodies and immunoblotted with indicated antibodies.