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. 2022 Aug 1;30(11):3414–3429. doi: 10.1016/j.ymthe.2022.07.019

Figure 5.

Figure 5

USP19 mimics the role of survivin in mitosis

(A) Mock, USP19KO, and USP19KO cells transfected with USP19 WT or USP19CA. Immunofluorescence staining was performed using phospho-histone H3 antibody. Scale bar, 100 μm. (B) The cells expressing GFP (phospho-histone H3+) were counted and presented graphically. Data are presented as the means and standard deviations of 3 independent experiments. One-way ANOVA followed by Tukey’s post hoc test was used with the indicated p values. (C) Mock, USP19KO, and USP19KO cells transfected with USP19 WT or USP19CA were synchronized by treating with 100 ng/mL nocodazole for 18 h. Western blot analysis was performed with the indicated antibodies. (D) Mock, USP19KO, and USP19KO cells transfected with USP19 WT or USP19CA were synchronized in prometaphase by treating with 100 ng/mL nocodazole for 18 h and then treated with MG132 for 4 h before harvesting. Western blot analysis was performed using indicated antibodies.