Figure 6.

USP19 and survivin expression profiles are positively correlated

(A) Heatmap showing the top 100 mRNA expression levels of USP19 and survivin derived from the DepMap portal. Representative samples are arranged from high to low mRNA levels of USP19, and corresponding survivin values are adjusted. (B) Scatterplot between USP19 and survivin mRNA levels from several cancer cell lines. Pearson correlation was performed to estimate the relationship between USP19 and survivin. (C) The mRNA expression profiles of USP19 and survivin were obtained from the GEPIA 2 database and graphically represented using Plotly to assess the correlation between USP19 and survivin. (D) Endogenous protein expression patterns of USP19 and survivin in different cell lines were determined by western blot analysis. In MCF7, MG63, and hDPSCs, the expression of USP19 showed lower molecular weight than the expected size. (E) The mRNA expression data of USP19 was obtained from the GENT2 database and represented graphically. Due to the small sample size for the expression of USP19 in the normal groups obtained from the GENT2 database, the statistical significance between normal and cancer groups could not be calculated. (F and G) Representative immunohistochemical staining images of endogenous USP19 and survivin in human breast cancer (n = 21) and colon cancer (n = 32). All immunohistochemistry (IHC) images were quantified by an H score. A 2-tailed t test was used, and p values are as indicated. Scale bar, 25 μm.