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. 2022 Aug 3;34(10):2345–2353. doi: 10.1007/s40520-022-02201-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Flow cytometric analysis of NETs DNA size. A Representative fluorescence histograms relative to DNA presents in NETS obtained from adult and elderly subjects. DNA was staining with SYBR Green dye (10 μg/ml) and green fluorescence obtained was displayed in a logarithmic scale. Region R1 was created to include the majority (more than 70%) of fluorescence obtained in adult samples; in samples from elderly subjects, DNA showed a different fluorescence distribution with a population showing higher fluorescence and, therefore, higher size (collected in Region R2) compared to adult samples. To stress the shift in DNA size during the aging, an overlapped histogram was added (adult/elderly). B Quantification of fluorescence events distribution between R1 and R2 in adult and elderly groups expressed as % of total events. ***p < 0.001 referred to R1