Figure 2.

Time course of PTH actions in mouse kidney near the Cyp27b1 gene.A, gene expression in the kidney for Cyp27b1 in 8- to 9-week-old WT C57BL/6 mice injected with 230 mg/kg bw PTH for 0, 1, 3, or 6 h. Data are displayed as relative quantitation (RQ, mean ± SEM) compared with Gapdh. n = 4 for each time point. One-way ANOVA with multiple comparison Tukey post test: ∗p < 0.05 time point versus 0 h. ChIP-Seq analysis near Cyp27b1 for (B) pCREB, (C) CBP, (D) CRTC2, (E) H3K27ac, or (F) H3K9ac from WT mice injected with 230 mg/kg bw PTH for 0 (Veh only), 15, 30, or 60 min (n = 3). Overlaid triplicate and averaged ChIP-Seq tracks where Veh (0 h) are shown in yellow, treatments shown in blue, and overlapping data appear as green. Regions of interest are highlighted in gray boxes (M1, CP [Cyp27b1 promoter], M21(a–c), tissue specific) or red (MP [Mettl1/Mettl21b promoters], nontissue specific). Genomic region displayed is chr10:126,469,260 to 126,490,800, and maximum height of tag sequence density for each data track indicated on the Y-axis (normalized to input and 10⁷ tags). Fold change table (right) was calculated for triplicate tag density in each peak region versus Veh. ∗p < 0.05 paired t test: treatment versus vehicle. nc, no change (<1.5-fold). bw, body weight; CBP, CREB-binding protein; ChIP-Seq, chromatin immunoprecipitation sequencing; CRTC, CREB-regulated transcription coactivator; H3K9ac, histone acetylation at histone H3 lysine 9; H3K27ac, histone acetylation at histone H3 lysine 27; pCREB, phosphorylated (p-133) CREB; PTH, parathyroid hormone.