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. 2022 Sep 30;298(11):102559. doi: 10.1016/j.jbc.2022.102559

Figure 3.

Figure 3

Time course of PTH actions in mouse kidney near the Cyp24a1 gene.A, gene expression in the kidney for Cyp24a1 in 8− to 9–week-old WT C57BL/6 mice injected with 230 mg/kg bw PTH for 0, 1, 3, or 6 h. Data are displayed as relative quantitation (RQ, mean ± SEM) compared with Gapdh. n = 4 for each time point. One-way ANOVA with multiple comparison Tukey post-test: ∗p < 0.05 time point versus 0 h. ChIP-Seq analysis near Cyp24a1 for (B) pCREB, (C) CBP, (D) CRTC2, (E) H3K27ac, or (F) H3K9ac from WT mice injected with 230 mg/kg bw PTH for 0 (Veh only), 15, 30, or 60 min (n = 3). Additional details as for Figure 2. Genomic region displayed is chr2: 170,278,379 to 170,324,235. bw, body weight; CBP, CREB-binding protein; ChIP-Seq, chromatin immunoprecipitation sequencing; CRTC, CREB-regulated transcription coactivator; H3K9ac, histone acetylation at histone H3 lysine 9; H3K27ac, histone acetylation at histone H3 lysine 27; pCREB, phosphorylated (p-133) CREB; PTH, parathyroid hormone.