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. 2022 Sep 30;298(11):102559. doi: 10.1016/j.jbc.2022.102559

Figure 8.

Figure 8

Activities of 1,25(OH)2D3and FGF23 at Cyp24a1. Gene expression of Cyp24a1 after time course with (A) 10 mg/kg bw 1,25(OH)2D3 at 0, 1, 3, 6, 12, and 24 h (n = 4) and (B) 50 mg/kg bw FGF23 at 0, 3, 6, and 12 h (n = 3–4) from our previous publication (10). ChIP-Seq analysis near Cyp24a1 for (C) VDR and RXR (1,25(OH)2D3versus Veh only), (D) pCREB, (E) CBP, (F) CRTC2, (G) H3K27ac, and (H) H3K9ac from WT mice injected with 10 mg/kg bw 1,25(OH)2D3 or 50 mg/kg bw FGF23 (1 h). Additional details as for Figure 2. Genomic region displayed is chr2: 170,278,379 to 170,324,235. bw, body weight; CBP, CREB-binding protein; ChIP-Seq, chromatin immunoprecipitation sequencing; CRTC2, CREB-regulated transcription coactivator 2; FGF23, fibroblast growth factor 23; H3K9ac, histone acetylation at histone H3 lysine 9; H3K27ac, histone acetylation at histone H3 lysine 27; 1,25(OH)2D3, 1,25-dihydroxyvitamin D3; pCREB, phosphorylated (p-133) CREB; RXR, retinoid X receptor; VDR, vitamin D receptor.