Figure 4.

ADV-CRISPR-SaCas9-mediated MECOM depletion inhibits tumor growth in LUSC PDX models

(A and B) Three types of viruses (ADV, 1 × 10¹⁰ VP; lentivirus, 1 × 10⁹ VP; AAV2, 1 × 10¹² VP) expressing GFP were intratumorally injected into the LUSC021 PDX model (NOD-SCID mice). The representative images (A) and infection efficiencies (B) of ADV, lentivirus, and AAV2 in the tumors isolated from those mice treated with different viruses by flow cytometry detection.

(C–F) A diagram (C) for intratumoral administration of ADV-CRISPR-SaCas9-mediated MECOM depletion in PDX models (NOD-SCID mice). The ADV system was intratumorally injected at days 0 and 12 with the amounts of 1.0 × 10¹⁰ VP and 0.5 × 10¹⁰ VP, respectively. Tumor growth curves and representative tumor photos of PDX models including LUSC006 (D), LUSC018 (E), and LUSC021 (F) treated with MECOM-depleted ADV (MECOM-KO) or control ADV. Scale bars, 1 cm. n = 5. ∗p ＜ 0.05, unpaired 2-tailed t test.