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. Author manuscript; available in PMC: 2023 Nov 3.
Published in final edited form as: Mol Cell. 2022 Oct 19;82(21):3985–4000.e4. doi: 10.1016/j.molcel.2022.09.026

Figure 1. TERRA contributes to the RAD52-independent ALT pathway.

Figure 1.

(A) A schematic introducing the bifurcated ALT pathway and the question on the role of TERRA in RAD52-independent ALT. (B-C) U2OS WT (B) and RAD52 KO (C) cells transfected with Control or TERRA LNA were analyzed by DRIP coupled with telomere-specific or β-actinspecific qPCR. Error bars: SEM, n=3 (experimental triplicates); ****: P value <0.0001; ***: P value 0.0001; NS: not significant. (D) The experimental scheme to visualize telomere DNA synthesis at APBs in G2 cells (left). U2OS WT and RAD52 KO cells were transfected with Control or TERRA LNA and synchronized in G2. The EdU signals at APBs were quantified in individual cells (n>120) and normalized by nuclear areas. Red lines: mean values. ****: P value <0.0001. (E) U2OS WT and RAD52 KO cells transfected with Control or TERRA LNA were analyzed by the C-circle assay. The RCA+/RCA-ratios of samples reflect the relative levels of C-circle amplification. Error bars: SEM, n=3 (experimental triplicates); **: P value 0.002, *: P value 0.02.