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. 2022 Oct 31;22:551–566. doi: 10.1016/j.bioactmat.2022.10.024

Table 3.

Trigger mechanism for hypervesiculation of bacterial outer membrane vesicles (OMVs).

Triggers for Vesiculation Mechanism Reference
Phenanthrene and hexadecane Hydrophobic carbon sources [186,187]
Toluene intercalates into the hydrophobic region and changes the membrane curvature [188]
Misfolded proteins like OmpA decreased location envelope ties between peptidoglycan and outer membrane lacking cross-linking components [53]
Lack of Lpp (abundant lipoprotein of E. coli)), L,D-transpeptidases YcfS, ErfK, degP, degS, fliC, flgK, glnA, lysS/herC, nlpA, nlpI, ompC, ompR, pepP, pnp, ponB, rmpM, rseA, tatC, wag/rfaG, wzxE, yieM, ypjM, LpxL2, LpxM, htrB, waaN, LpxL, msbB, waaM, LpxL1, msbB and YbiS Alters the cell envelope [53,91,131]
Mutation of peptidoglycan amidase or increase in peptidoglycan hydrolyzing enzymes increase the outer membrane material [131,189]
Genetic modulation like null Nlpl, increased expression of N-terminal domain of g3p phage protein, domains of colicins A and E3, LpxR, pagL, LpxO, Hp0021, msbB gene Upregulation or remodeling of necessary cell envelope lipids [91,99,190,191]
Increase in lactate source of carbon [153]
EGTA, a calcium chelator depletes the calcium needed to maintain the crystalline surface of bacilli [192]
Regulating spore formation pathway - [193]