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. Author manuscript; available in PMC: 2023 Nov 3.
Published in final edited form as: Mol Cell. 2022 Sep 30;82(21):4049–4063.e6. doi: 10.1016/j.molcel.2022.09.002

Figure 2. Dcr-1•Loqs-PB conformational equilibrium strongly favors closed states.

Figure 2.

(A) Overall views of the RNA-free Dcr-1•Loqs-PB (structure Ia) and pre-miRNA-bound Dcr-1•Loqs-PB in presence of Ca2+ (structure IIa).

(B–D) Close-up views showing differences in the positions of the Dcr-1 dsRBD (B), the Dicer-specific α-helix of the PAZ domain (C), and the Dcr-1 Hel2i and the Loqs-PB dsRBD-3 domains (D) between the RNA-free structure Ia (colored) and the RNA-bound structure IIa (protein domains in dark gray and pre-miRNA in gray). Structural alignments were performed by superposition of Dcr-1•Loqs-PB complexes. Black arrows highlight conformational changes upon pre-miRNA binding.

See also Figure S5.