FIG. 5.

In situ hybridization of whole-mount pyocin particles with a complementary probe to pyocin DNA. The in situ hybridizations were performed as outlined in Materials and Methods. The gold beads were visible in association with the particles only when probed with the antisense probe. The gold beads were found in the core and on the sheath. In some instances, they appeared at one end of the pyocin particle. This indicates tight, specific interaction between the DNA and the particle. Bar, 100 nm.