TABLE 1.
Bacterial strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotides | Genotype, markers, sequence | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F-, thi-1 f80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk− mk+) supE44 gyrA96 relA | Bethesda Research Laboratories—Life Technologies |
| E. coli NM522 | F′, lacIq Δ(lacZ)M15 proA+ B+ supE thi Δ(lac-proAB) Δ(hsdMS-mcrB)5 (rk− mk+) mcrBC | New England Biolabs |
| N. gonorrhoeae 1291 | Our collection | |
| P. aeruginosa C | 14 | |
| P. aeruginosa PAO-1 | 35 | |
| Plasmids | ||
| pGEM3zf+ | Ampicillin resistant | Promega Corp. |
| pPSC-1 | Ampicillin resistant | This study |
| pPSC-2 | Ampicillin resistant | This study |
| pPSC-3 | Ampicillin resistant | This study |
| pPSC-4 | Ampicillin resistant | This study |
| Oligonucleotides | ||
| T7 primer | Promega Corp. | |
| SP6 primer | Promega Corp. | |
| PSC-3 OLIGO 1 | 5′-ATG GCT CTT GAT AGT TCG GG-3′ | |
| PSC-3 OLIGO 2 | 5′-CCC GAA CTA TCA AGA GCC AT-3′ | |
| A12 SP6-EXT | 5′-AGT TTC AGC GCG TTG AGT-3′ | |
| A12 SP6-OLIGO 1 | 5′-CGG GAT GAT CTT TAT CGG AA-3′ |