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Iridium preparation
| 1 sample | 11 samples (1 acquisition batch) | 48 samples (4 acquisition batches) | Total volume needed (+ ∼20%) | |
|---|---|---|---|---|
| Number of cells / sample | 1.5 × 106 cells | 16.5 × 106 cells | 66 × 106 cells | |
| Staining volume | 150 μL | 1,650 μL | 7,200 μL | 8,640 μL |
| Ir (stock 500 μM) | 0.0375 μL | 0.4125 μL | 1.8 μL | 2.16 μL |
Preparation of Ir solution for one sample and scaled to 1 batch and a multibatch experiment (4 batches).
