Skip to main content
. 2022 Sep 23;298(11):102522. doi: 10.1016/j.jbc.2022.102522

Figure 2.

Figure 2

Enzymatic characterization of IPCS activity and its absence in Δipcs−promastigotes. A, NBD-IPC formation in complete reactions or in the absence of key substrates, microsomes, inhibitors (20 μM aureobasidin A), or enzymatic treatments, as indicated. Statistical comparisons to the standard reaction (column 1) are shown; ∗∗p <0.02; ∗∗∗p< 0.0001 (ANOVA with Dunnet’s post hoc analysis). B, quantitation of NBD-IPC formation by microsomes from WT, Δipcs−, or Δipcs−/+IPCS parasites. ∗∗∗, p < 0.0001 (ANOVA with Tukey’s post hoc analysis). For both panels, primary TLC data can be found in Fig. S1, three experiments with two technical replicates were performed, and bars represent the mean ± 1 SD. IPCS, inositol phosphorylceramide synthase.