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. 2022 Sep 23;298(11):102522. doi: 10.1016/j.jbc.2022.102522

Figure 4.

Figure 4

Phenotypic characterization of Δipcs−promastigotes in vitro.A, growth, and (B) propidium iodide exclusion (viability), seven experimental replicates (C), growth in the presence of 2 μM 3-keto-dihydrosphingosine (⊗), dihydrosphingosine (◐) or phytosphingosine (◒; n = 1 for each) and; (D) growth at pH 5.0 (MES buffer), two experimental replicates. All data points are mean ± 1SD, ANOVA tests for each panel were significant (p ≤ 0.002) followed by Bonferroni correction to adjust for multiple comparisons, ∗ post hoc comparisons between WT and Δipcs− significant at p <0.05, ∗∗ post hoc comparisons between Δipcs−/+IPCS and Δipcs− significant at p <0.05. All parasites were propagated in standard M199 culture media at 25 °C. Lines tested are WT (•, solid line), Δipcs− (○), or Δipcs−/+IPCS (▼, dashed line). IPCS, inositol phosphorylceramide synthase.