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. 2022 Sep 23;298(11):102522. doi: 10.1016/j.jbc.2022.102522

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

ESI/MS analysis (positive ion mode) of SMs from purified amastigotes and infected tissues. Spectra were acquired in the positive ion mode showing the [M + Na]+ ions of SM (red) and PC, PE, or TG (black). Ions labeled in green are spike-in controls (d18:1/12:0 SM or 14:0/14:0-PC). Lipids were prepared from (A) purified WT amastigotes, (B) purified Δipcs⁻ amastigotes (clone WAE), and (C) total WT infected footpad lesions. γKO host mice were used in the studies shown; similar findings with BALB/c hosts are shown in Fig. S8. d18:1/16:0-SM, N-palmitoyl-octadecesphing-4-enine-1-phosphocholine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; SM, sphingomyelin; TG, triacylglycerol.. For phospholipid (PL) designation: A/B-PL illustrates A: combined number of carbon chain of the fatty acid (FA) attached to glycerol backbone; B: total number of unsaturated bonds of FA substituents. ESI/MS, electrospray ionization mass spectrometry.