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. 2022 Sep 23;298(11):102522. doi: 10.1016/j.jbc.2022.102522

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Relative abundance of WT amastigote SM and IPC. Internal standards (10 nmol d18:1/12:0-SM and 2 nmol of dipalmitoyl phosphoinositol) were added to WT amastigote samples, and lipids were extracted and analyzed by ESI/MS in the positive ion mode (panel A) or negative ion mode (panel B) to compare the relative amounts of SM and IPC present in the same sample. This experiment was repeated a second time with similar results1. ESI/MS, electrospray ionization mass spectrometry; IPC, inositol phosphorylceramide; SM, sphingomyelin.