FIG. 4.

Changes in the expression of adhesion molecules on monocytes incubated with Y. enterocolitica O:3, S. pyogenes, or LPS of Y. enterocolitica O:3. (A) Y. enterocolitica O:3 downregulates the expression of CD11a but not the expression of β1-integrins VLA-α4 and VLA-α5. (B) The effect of Y. enterocolitica on the expression of β2-integrins seemed to be at least partially mediated by LPS. (C) S. pyogenes was able to slightly reduce the expression of α4 and α5 integrins. Incubation time refers to the length of time that the monocytes were allowed to process the bacteria. Results are counted as net mean fluorescence intensity (MFI) values (negative control value subtracted from the experimental value) ± standard errors of the means and expressed as relative mean fluorescence intensities, in which the mean fluorescence intensity of the control monocytes was given the value 1.0. ∗, P < 0.05; ∗∗, P < 0.005.