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. 2022 May 31;39(9):110889. doi: 10.1016/j.celrep.2022.110889

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© 2022 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

JAZF1-SUZ12 lacks interaction with JARID2, EPOP, and PALI1 due to loss of the SUZ12 N terminus

(A) Models of FLAG-tagged (F) SUZ12, SUZ12Δ93, JAZF1-SUZ12, and JAZF1 constructs.

(B) Immunoblots for FLAG, SUZ12 (N terminus), EZH2, JARID2, AEBP2, PCL2, EPOP, TRRAP, and β-actin in input (In.) and SUZ12 IPs from WT or Suz12^GT/GT ESCs stably expressing FLAG-tagged GFP, SUZ12, SUZ12Δ93, JAZF1-SUZ12, or JAZF1. Data are representative of two independent IPs.

(C) Immunoblots for FLAG, HA-PALI, and EZH2 in input and Strep-Tactin pull-downs (P) from NIH3T3 cells transfected with HA-PALI1 and FS2-tagged GFP, SUZ12, SUZ12Δ93, JAZF1-SUZ12, or JAZF1. Data are representative of two independent pull-downs.

See also Figure S1.