Figure 4.

JAZF1-SUZ12 disrupts gene expression during cell differentiation

(A) Expression of Oct4, Nanog, Utf1, and Fgf4 (relative to Gapdh and day 0) at 0, 4, and 8 days after initiation of EB formation in Suz12^GT/GT ESCs expressing GFP, SUZ12, SUZ12Δ93, or JAZF1-SUZ12 (mean and SD of four independent EB inductions; ^∗p < 0.05; two-way ANOVA).

(B) Expression of Pax3, T, Fgf5, and Gata4 (relative to Gapdh; other details as for A).

(C) Size of EBs formed by WT and Suz12^GT/GT ESCs expressing GFP, SUZ12, SUZ12Δ93, or JAZF1-SUZ12 at days 2, 4, 6, and 8 (mean and SD; n ≥ 55 EBs; ^∗∗∗∗p < 0.0001; two-way ANOVA).

(D) Representative images of EBs from the time course described in (C). Scale bars represent 0.65 mm.