REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FLAG (M2)	Sigma	Cat# F3165; RRID:AB_259529
HA (3F10)	Roche	Cat# 12013819001; RRID: AB_390917
V5	Abcam	Cat# ab15828; RRID: AB_443253
FLAG-HRP	Sigma	Cat# P1379
SUZ12	Santa Cruz	Cat# sc-46264; RRID: AB_2196857
EZH2	Cell Signaling Technology	Cat# 3147; RRID: AB_10694383
JARID2	Cell Signaling Technology	Cat# 13594; RRID: AB_2798269
AEBP2	Cell Signaling Technology	Cat# 14129; RRID: AB_2798398
PCL2	Proteintech	Cat# 16208-1-AP; RRID: AB_2147370
EED	A. Bracken	Cat# 05–1320; RRID: AB_1586999
EPOP	L. Di Croce	Beringer et al. (2016)
TRRAP	Abcam	Cat# ab73546; RRID:AB_10672042
Alpha-tubulin	Cell Signaling Technology	Cat# 2144; RRID: AB_2210548
Beta-actin	Cell Signaling Technology	Cat# 4967; RRID:AB_330288
Histone H3	Abcam	Cat# ab1791; RRID:AB_302613
H3K27me3	Abcam	Cat# ab192985; RRID: AB_2650559
H4 pan-acetyl (K5,K8,K12)	Thermofisher	Cat# PA5-40083; RRID: AB_2608361
Biological samples
Endometrial biopsies for primary human endometrial stromal cells	University Hospitals Coventry and Warwickshire (UHCW) NHS Trust, Implantation Clinic Coventry	https://www.tommys.org/research/research-centres/tommys-national-reproductive-health-biobank
Chemicals, peptides, and recombinant proteins
Estradiol	Sigma	Cat# E2758
Insulin	Santa Cruz	Cat# sc-360248
Benzonase	Sigma	Cat# E1014
Leukemia inhibitory factor (LIF)	Stemgent	Cat# 03-0011-100
Polybrene	Merck	Cat# TR-1003-G
Medroxyprogesterone acetate (MPA)	Sigma	Cat# M1629
8-Br-cAMP	Merck	Cat# B5386
Polyethylenimine (PEI)	Polysciences	Cat# 23966
DNase Turbo	Ambion	Cat# AM2238
Dynabeads protein G	Invitrogen	Cat# 10003D
Streptactin high-capacity resin	IBA Life Sciences	Cat# 2-1208-002
Avidin	IBA Life Sciences	Cat# 2-0204-015
TRIsure	Bioline	Cat# BIO-38033
RNaseA	Sigma	Cat# R6513
Effectene	Qiagen	Cat# 301425
Kapa Pure beads	Roche	Cat# KK8000
DNase Turbo	Ambion	Cat# AM2238
Critical commercial assays
NEBNext Ultra II DNA library preparation kit	NEB	Cat# E7645
Clarity ECL	Biorad	Cat# 1705061
ImProm-II Reverse Transcription System	Promega	Cat# A3800
KAPA Stranded RNA-Seq Kit with RiboErase	Roche	Cat# KR1151
KAPA Universal Library Quantification kit	Roche	Cat# KK4824
QuantiTect SYBR Green PCR kit	Qiagen	Cat# 204143
2100 Bioanalyzer High Sensitivity DNA kit	Agilent	Cat# 5067–4626
Agilent RNA 6000 Pico kit	Agilent	Cat# 5067–1513
Qubit dsDNA HS assay kit	Thermofisher	Cat# Q32851
Qubit RNA High Sensitivity assay kit	Thermofisher	Cat# Q32855
Deposited data
ChIP-seq data	This paper	GEO: GSE169658
RNA-seq data	This paper	GEO: GSE169658
Western Blot raw image files	This paper	Mendeley Data: https://doi.org/10.17632/52rbsgb6by.1
Experimental models: Cell lines
E14 ESC	Hooper et al. (1987)	RRID:CVCL_C320
Suz12GT/GT ESC	Pasini et al. (2007)	N/A
NIH3T3	B. Vanhaesebroeck	RRID:CVCL_0594
Lenti-X 293T	Takara Biosciences	RRID:CVCL_4401
hTERT immortalised fibroblasts	Scaffidi and Misteli (2011)	N/A
Drosophila S2 cells	I. Bjedov	RRID:CVCL_Z232
Oligonucleotides
Primers for RT-qPCR, see Table S3 provided	This work	N/A
Recombinant DNA
pCAG-GFP-FLAG	A. Fisher	N/A
pCAG-SUZ12-FLAG	This work	N/A
pCAG-SUZ12Δ93-FLAG	This work	N/A
pCAG-JAZF1-SUZ12-FLAG	This work	N/A
pCAG-JAZF1-FLAG	This work	N/A
pCAG-FS2-GFP	This work	N/A
pCAG-FS2-SUZ12	This work	N/A
pCAG-FS2-SUZ12Δ93	This work	N/A
pCAG-FS2-JAZF1-SUZ12	This work	N/A
pCAG-FS2-JAZF1	This work	N/A
pMY-GFP-FLAG-IRES-bls	This work	N/A
pMY-SUZ12-FLAG-IRES-bls	This work	N/A
pMY-SUZ12Δ93-FLAG-IRES-bls	This work	N/A
pMY-JAZF1-SUZ12-FLAG-IRES-bls	This work	N/A
pMY-JAZF1-FLAG-IRES-bls	This work	N/A
pCBA-SUZ12-HA	This work	N/A
pCBA-SUZ12Δ93-HA	This work	N/A
pCBA-JAZF1-SUZ12-HA	This work	N/A
pCBA-JAZF1-HA	This work	N/A
pCAG-FS2-AEBP2	Grijzenhout et al. (2016)	N/A
pCAG-FS2-EPOP	Grijzenhout et al. (2016)	N/A
pLenti-FLAG-HA-PALI1	Conway et al. (2018)	N/A
PCL3-V5	Hunkapiller et al. (2012)	N/A
pCMV-Gag-Pol	G. Towers	Cat# RV-111
pMDG	G. Towers	N/A
Software and algorithms
Ensembl BioMart	Smedley et al. (2009)	RRID:SCR_010714
Graphpad Prism version 8.0	GraphPad	RRID:SCR_002798
ImageQUant TL software	GE Life Sciences	RRID:SCR_014246
ImageJ version 1.50i	National Institutes of Health	RRID:SCR_003070
bowtie2 version 2.1.0	Langmead and Salzberg (2012)	RRID:SCR_016368
Bedtools	Quinlan and Hall (2010)	RRID: SCR_006646
DiffBind	Ross-Innes et al. (2012)	RRID:SCR_012918
MACS2	Feng et al. (2012)	RRID: SCR_013291
UpSetR	Conway et al. (2017)	N/A
Trim Galore	Babraham Institute	RRID:SCR_011847
STAR version 2.7.3a	Dobin et al. (2013)	RRID:SCR_004463
DeepTools version 3.0.2	Ramírez et al. (2014)	RRID:SCR_016366
featureCounts version 5.25	Liao et al. (2014)	RRID:SCR_012919
DEseq2	Love et al. (2014)	RRID:SCR_015687
ggplot2	Wickham (2016)	RRID:SCR_014601
Pheatmap version 1.2	Kolde (2012)	RRID:SCR_016418
EnhancedVolcano	Blighe et al. (2019)	RRID:SCR_018931
g:Profiler2 v1.2	Raudvere et al. (2019)	RRID:SCR_018190
Fgsea	Korotkevich et al., (2021)	RRID:SCR_020938
R version 3.6.1	R Core Team (2019)	RRID:SCR_001905