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. 2022 May 31;39(9):110903. doi: 10.1016/j.celrep.2022.110903

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© 2022 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Accumulation of GMAP in Atg8a^KG07569 and Atg8a^K48A/Y49A mutant flies

(A) Western blot analysis shows that GMAP is accumulated in both Atg8a ^KG07569 and Atg8a^K48A/Y49A mutant flies.

(B) Quantification of GMAP in Atg8a^KG07569 and Atg8a^K48A/Y49A mutant flies.

(C) Confocal images from 2-week-old adult brains, GMAP (green) (arrows) and ubiquitin (red) aggregates can be seen in Atg8a^KG07569 and Atg8a^K48A/Y49A mutant flies. DNA was dyed with Hoechst (blue). Scale bars, 10 μm.

(D) Average GMAP puncta size is larger in Atg8a^KG07569 and Atg8a^K48A/Y49A mutant flies compared with wild-type flies. Bar charts show means ± SD. Statistical significance was determined using two-tailed Student’s t test. ^∗p < 0.05. Number of biological repeats (N = 3 for all figures). Genotypes for all figures: wild-type: w¹¹¹⁸/Y, Atg8a LDS: Atg8a ^K48A/Y49A/Y, Atg8a: Atg8a ^KG07569/Y.