Figure 5.

GMAP regulates Golgi turnover via autophagy

(A) Confocal images showing co-localization of endogenous Atg8a and the GMAP under starvation conditions in adult fat body in control and GMAP ^F322A/V325A mutant flies.

(B) Western blots showing accumulation of the Golgi marker GM130 in Atg8a ^KG07569 and Atg8a^K48A/Y49A mutant flies compared with wild-type flies.

(C) Western blots showing accumulation of GM130 in GMAP-RNAi lines compared with control RNAi and its quantification shown below.

(D) Western blots showing accumulation of GM130 in GMAP ^F322A/V325A mutant flies compared with wild-type flies.

(E) Immunofluorescence confocal microscopy of Drosophila brain showing increased accumulation of the cis-Golgi marker (GM130) and the altered morphology of Golgi in GMAP ^F322A/V325A and Atg8a ^KG07569 mutant flies. Scale bars, 10 μm.

(F) Average GM130 puncta size is larger in GMAP ^F322A/V325A and Atg8a ^KG07569 mutant flies compared with wild-type flies. Bar charts show means ± SD. Statistical significance was determined using two-tailed Student’s t test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Number of biological repeats (N = 3 for all figures). Genotypes: (A) Control: w¹¹¹⁸/Y, GMAP: GMAP ^F322A/V325A/Y. (B) Control: w¹¹¹⁸/Y, Atg8a LDS: Atg8a ^K48A/Y49A/Y, Atg8a: Atg8a ^KG07569/Y. (C) Control: yw¹¹¹⁸;P{attP,y[+],w[3`]}/+;da-GAL4/+, GMAP-RNAi: GMAP-RNAi/+; da-GAL4/+. (D and E) Control:w¹¹¹⁸/Y, GMAP: GMAP ^F322A/V325A/Y, Atg8a: Atg8a ^KG07569/Y.