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. 2022 Jul 5;40(1):111037. doi: 10.1016/j.celrep.2022.111037

Figure 1.

Figure 1

The three main subclasses of Htr3a+ interneurons undergo programmed cell death

(A) Coronal sections through the primary somatosensory cortex of control and Htr3a-Cre;Bak−/−;Baxfl/fl mice at P30 following immunohistochemistry against Prox1 (cyan), reelin (magenta), and calretinin (yellow). DAPI is shown for counterstaining (grey).

(B) Quantification of the density of all Htr3a+ interneurons (Prox1+), neurogliaform cells (Prox1+ and Reln+), bipolar cells (Prox1+ and CR+), and basket cells (Prox1+, Reln−, and CR−) in control (gray boxplots, n = 5 mice) and Htr3a-Cre;Bak−/−;Baxfl/fl mice (green boxplots, n = 4 mice) at P30. Prox1+: two-tailed unpaired Student’s t test, ∗p = 0.01. Prox1+ Reln+: two-tailed unpaired Student’s t test, ∗p = 0.03. Prox1+ and CR+: two-tailed unpaired Student’s t test, ∗p = 0.03. Prox1+, Reln−, and CR−: two-tailed unpaired Student’s t test, ∗p = 0.03.

Data in (B) are shown as boxplots (median, middle dash), lower and upper quartiles (box borders), and minimum and maximum (whiskers), and the adjacent data points indicate the average cell density in each animal. Scale bar, 100 μm. See also Figure S1.