Figure 5.

The survival of bipolar cells is independent of glutamate transmission

(A) Schematic of experimental design.

(B) Coronal sections through the primary motor cortex of control and Vglut1^−/−;Vglut2^fl/fl mice at P21 following immunohistochemistry against Prox1 (cyan), GFP (green), and calretinin (yellow). DAPI is shown for counterstaining (gray).

(C) Quantification of the density of all Htr3a+ interneurons (Prox1+), neurogliaform cells and basket cells (Prox1+ and CR-), and bipolar cells (Prox1+ and CR+) in control (gray boxplots, n = 4 mice) and Vglut1^−/−;Vglut2^fl/fl mice (green boxplots, n = 4 mice) at P21. Prox1+: two-tailed unpaired Student’s t test, p = 0.10. Prox1+ and CR−: two-tailed unpaired Student’s t test, ^∗p = 0.02. Prox1+ and CR+: two-tailed unpaired Student’s t test, p = 0.99.

Data in (C) are shown as boxplots (median, middle dash), lower and upper quartiles (box borders), and minimum and maximum (whiskers), and the adjacent data points indicate the average cell density in each animal. Scale bar, 100 μm. See also Figures S1 and S4.