Figure 7.

The survival of bipolar cells depends on monoamine transmission from pyramidal cells

(A) Schematic of experimental design.

(B) mCerulean (top) and mCherry (bottom) expression at P21 following AAV injections at P0.

(C) Coronal sections through the primary somatosensory cortex of Nex^Cre/+ mice at P21 injected with hM3Dq-mCherry and shLacZ-mCerulean (left) or shSlc18a2-mCerrulean (right) virus followed by CNO treatment immunostained for Prox1 (cyan) and calretinin (yellow).

(D) Quantification of the density of all Htr3a+ interneurons (Prox1+), neurogliaform cells and basket cells (Prox1+ and CR-), and bipolar cells (Prox1+ and CR+) in shLacZ-mCerulean (gray boxplots, n = 4 mice) and shSlc18a2-mCerrulean mice (green boxplots, n = 4 mice) at P21. Prox1+: two-tailed unpaired Student’s t test, p = 0.53. Prox1+ and CR−: two-tailed unpaired Student’s t test, p = 0.85. Prox1+ and CR+: two-tailed unpaired Student’s t test, ^∗p = 0.03.

Data in (C) are shown as boxplots (median, middle dash), lower and upper quartiles (box borders), and minimum and maximum (whiskers), and the adjacent data points indicate the average cell density in each animal. Scale bar, 100 μm. See also Figures S1, S6, and S7.