Figure 2.

Clinico-pathology, subtype, and survival are related to an individual’s position on the group 3/group 4 continuum

(A) Rug plot showing distribution of clinico-pathological features with respect to G3/G4 score. Summary counts are given according to the divisions of highG4, lowG4, G3.5, lowG3, and highG3 (these categories are arbitrary divisions of the continuum for the purposes of visualization and comparison and do not represent “real” subgroups) and reflected by the red line plots. The presence of a feature is indicated by a bold tick mark, the color of which indicates MB_Grp3/MB_Grp4 methylation subtype (I–VIII). Adjusted p values for a Kolmogorov-Smirnoff statistic (D) are shown to denote non-random distribution of features with respect to G3/G4 score. Mismatch, mismatch between methylation and expression call; Infant, age at diagnosis younger than 3 years; M+, metastatic; DOD, dead of disease; LCA, large-cell/anaplastic; PRDM6, PRDM6 rearrangement.

(B) Kaplan-Meier plot showing significant differences (Log-Rank test for trend) in MB_Grp3/MB_Grp4 overall survival by G3/G4 continuum position.

(C) Forest plot showing a multivariate Cox model fitted to progression-free survival and containing the independently significant variables highG3, MYC amplification, LCA, and M+.

(D) Violin plot showing G3/G4 score (derived from methylation) by MB_Grp3/MB_Grp4 (I–VIII) subtype.

(E) Kaplan-Meier plot showing significant differences (Log-Rank test for trend) in MB_Grp3/MB_Grp4 overall survival in patients aged older than 3 years by G3/G4 score (as derived from methylation values); n = 589.