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. 2022 Nov 5;31(3):870–885. doi: 10.1007/s10924-022-02615-x

Table 3.

Primers used in qRT-PCR

B2M NM_004048.4 Homo sapiens beta-2-microglobulin (B2M)
Forward primer CCACTGAAAAAGATGAGTATGCCT 126 bp 60 °C
Reverse primer CCAATCCAAATGCGGCATCTTCA
RUNX2 NM_001278478.2 Homo sapiens RUNX family transcription factor 2 (RUNX2)
Forward primer TAGGCGCATTTCAGGTGCTT 105BP 60 °C
Reverse primer TGCATTCGTGGGTTGGAGA
Osteocalcin OC NM_199173.6 Homo sapiens bone gamma-carboxyglutamate protein (BGLAP)
Forward primer CCTCACACTCCTCGCCCTATT 250 bp 60 °C
Reverse primer GGTCAGCCAACTCGTCACA
Osteopontin OP NM_000582.3 Homo sapiens secreted phosphoprotein 1 (SPP1)
Forward primer GCCGAGGTGATAGTGTGGTT 149 bp 60 °C
Reverse primer AACGGGGATGGCCTTGTATG